Institute of Medical Psychology
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Systems Chronobiology-Robles Lab

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Research:

The circadian clock is an endogenous timing system that regulates in a daily manner physiological functions and behavior by modulating gene expression and protein function. Circadian control of transcription regulation is widely studied however the understanding of circadian dynamics in protein function is in its infancy. My work in the last years has pioneered the use of mass spectrometry-based quantitative proteomics to study temporal dynamics of protein abundance, cellular localization and post-transcriptional modifications (PTMs). This technology has thus proven instrumental in chronobiology to comprehensively understand post-translational mechanism regulating circadian metabolism and physiology. Our group has acquired a state of the art instrument (Q-Exactive™ HF-X) located at the BMC building (https://www.bmc.med.uni-muenchen.de/index.html) that allows us to study basic circadian molecular mechanism. In addition, our work also focuses in the interplay of metabolic, behavioral and cognitive disorders with circadian asynchrony, a condition frequently associated with modern life styles.

ongoing projects:

-The Fourth Dimension: Integration of time to shape co-operativity and survival in the biosphere

Life? Volkswagen Stiftung

http://portal.volkswagenstiftung.de/search/projectDetails.do?ref=93070

-Proteomics subtyping and circadian dynamics of PDACs to develop novel therapeutic strategies

SFB1321 Modelling and Targeting Pancreatic Cancer
https://www.sfb1321.med.tum.de/en

 

Group members:

Tanja Bange

Franziska Brüning

Stella Koutsouli

News and conferences:

https://www.proteinanalytik.abi.med.uni-muenchen.de/news/new-group-at-zfp/index.html

https://www.cas.uni-muenchen.de/schwerpunkte/microbiome/index.html

https://www.imb.de/seminars-meetings/meetings/imb-workshop-molecular-mechanisms-of-circadian-clocks/

https://www.grc.org/chronobiology-conference/2019/

Publications:

Korge, S, Maier, B, Brüning, F, Ehrhardt, L, Korte, T, Mann, M, Herrmann, A, Robles MS and Kramer A. (2018) The non-classical nuclear import carrier Transportin 1 modulates circadian rhythms through its effect on PER1 nuclear localization. PLoS Genet. In press

Hughes ME,… Robles MS,…, Hogenesch JB. (2017) Guidelines for genome-scale analysis of biological rhythms. J Biol Rhythms Oct;32(5):380-393.

Robles MS, Humphrey SJ and Mann, M (2017) Central role of phosphorylation in the circadian control of liver metabolism. Cell Metab Jan 10; 25(1):118-127.

Neufeld-Cohen A*, Robles MS*#, Adamovich Y, Nir D, Rousso-Noori L, Yael Kuperman Y, Golik M, Mann M and Asher G#. (2016) Circadian control of mitochondrial nutrient utilization through regulation of rate-limiting mitochondrial enzymes. PNAS, doi: 10.1073 *Equal contribution; #co-corresponding author

Tamayo AG, Duong HA, Robles MS, Mann M, Weitz CJ. (2015) Histone mono-ubiquitination by a Clock–Bmal1 complex marks circadian clock genes for feedback repression. Nature Structural and Molecular Biology, Oct; 22(10):759-66

Robles MS, Cox J, Mann M. (2014) In-vivo quantitative proteomics reveals a key contribution of post-transcriptional mechanisms to the circadian regulation of liver metabolism. PLoS Genet. Jan; 10(1): e1004047.
Robles MS, Mann M. (2013) Proteomic approaches in circadian biology. Handb Exp Pharmacol.; (217): 389-407.

Padmanabhan K, Robles MS, Westerling T, Weitz CJ. (2012) Feedback regulation of transcriptional termination by the mammalian circadian clock PERIOD complex. Science. Aug 3; 337 (6094): 599-602.

Duong HA*, Robles MS*, Knutti D, Weitz CJ. (2011) A molecular mechanism for circadian clock negative feedback. Science. Jun 17; 332 (6036): 1436-9. *Equal contribution

Robles MS*, Boyault C*, Knutti D*, Padmanabhan K, Weitz CJ. (2010) Identification of RACK1 and protein kinase C alpha as integral components of the mammalian circadian clock. Science. Jan 22; 327 (5964): 463-6. *Equal contribution

Robles MS, Leonardo EM, Izquierdo M and Martínez-A C. (2003) Exacerbated inflammatory responses in transgenic mice expressing an inhibitor of apoptosis protein (OpIAP). Cell Death and Differ. 10 (11): 1226-33

Robles MS, Leonardo E, Criado LM, Izquierdo M. and Martínez-AC. (2002) OpIAP provides a costimulatory signal required for optimal proliferation of developing thymocytes. Journal of Immunology, 168: 1770-1779.

Jobs:

We are looking for motivated PhD students to fill two currently open positions in our group. Although we do not have any available position for Postdocs, fellows who can attract their own funding are very welcome to apply.
Masters students are also welcome to inquire for internships/short-term projects in the lab. To apply or for more information please contact:

Charo Robles (charo.robles@med.lmu.de)


We are faculty members of the Molecular IMPRS for Molecular Life Sciences PhD program in Munich (https://imprs-ls.opencampus.net/en).

 


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